Assays that measure [3H] thymidine incorporation by cells plated in soft agar were investigated to identify a rapid method for assessing chemosensitivity of tumor cells.
Two established cell lines (HeLa and JAR) were tested for the effectivenss of chemotherapeutic agents using colony forming assay on plastic and assay that measure [3H] thymidine incorporation by scintillation, and the results was compared.
1. Colony forming efficiency of HeLa on plastic was 17.5%, and that of JAR was 18.9%.
2. By colony forming assay, drug-related inhibition(%) of colony formation of HeLa on Cisplatin, Vinblastine, Mitomycin, and Adriamycin was 71%, over 99%, over 99%, and 62%, and that of JAR was 83%, over 99%, 68%, and over 99%, respectively.
3. Drug-related inhibition (%) of [3H] thymidine incorporation by scintillation of HeLa on Cisplatin, Vinblastine, Mitomycin, and Adriamycin was 75%, over 99%, 96%, and 69%, and that of JAR was 34%, over 99%, 78%, and 88%, respectively.
4. There was no significant difference between two methods comparing chemosensitivity except JAR, which has significant difference between two methods on Cisplatin (P<0.005). (Inhibition of colony formation on plastic was 83%, where as
inhibition
of
[3H] thymidine incorporation by scintillation was 34%).
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